Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Biotang Inc Tricine-SDS Sample Buffer (2X), reducing, 25 ml
Tricine-SDS Sample Buffer (2X), reducing, 25 ml.
Sigma Aldrich Fine Chemicals Biosciences Trizma(R) base >=99.0% (T) | 77-86-1 | MFCD00004679 | 100G
Trizma(R) base >=99.0% (T) | Purity: >=99.0% (T) | Mol Wt: 121.14 | 77-86-1 | MFCD00004679 | 100G
Pfanstiehl Inc Tris Hydrochloride (Tris HCl) High Purity, Low Endotoxin, Low Metals (T-154) 1Kg GMP CAS: 1185-53-1 Injectable Excipient Grade
Pfanstiehl Tris HCL, High Purity, Low Endotoxin, Low Metals, is a buffer component that has been specifically designed and formulated to remove impurities such as endotoxin, trace metals, and nitrosamines. Manufactured in the USA under ICH-Q7 cGMP conditions, Pfanstiehl Tris HCl meets parenteral (injectable) excipient grade standards, making it ideal for pharmaceutical, biologic, and vaccine bioprocessing and formulation. cGMP , pharmaceutical grade .
AdipoGen Trishydroxymethyl aminometha
Chemical. CAS 77-86-1. Formula C4H11NO3. MW 121.14. Synthetic. A high quality buffer used in the range of physiological pH 7-9. Widely used as a biological buffer or as a component of buffer formulations. Tris has a pKa of 8.06 and is very useful in the biology and biochemistry lab because it has buffering capabilities in the range of the typical physiological pH of most living organisms pH 7.0-9.0. Tris has been reported to interfere with the activity of a number of enzymes, it should therefore be used carefully when studying proteins.
Bioworld PEB- Protein Extraction Buffer (5x), 500 mL
Tris Protein Extraction Buffer (PEB), 5X Lysis buffer for extracting proteins from cells. Technical Information:5X concentrationTris-HCL, pH 8.0 (0.5M)B-mercaptoethanol (100mM)EDTA (20mM)Add protease inhibitor according to your protocol
Teknova 10mM Tris-HCl, pH 8.5. 2mL, 100-Pack, Sterile. 2 pack.
Tris (tris(hydroxymethyl)aminomethane) is used as a component ofbuffer solutions, especially for solutions ofnucleic acids. The useful buffer range for tris (pH 7–9) is similar to the pH of most living organisms. This, and its low cost, make tris one of the most common buffers in the biology/biochemistry laboratory.
Biotang Inc TRIS-HCL Buffer 1.0M, pH 7.2 (0.2 micron filtered), 500 ml
TRIS-HCL Buffer 1.0M, pH 7.2 (0.2 micron filtered), 500 ml.
Teknova Elution Solution-10mM Tris-HCl pH 8.5, 0.01% Tween-20. 1000mL. DNAse Tested. Sterile. 8 pack.
Elution Solution-10mM Tris-HCl pH 8.5, 0.01% Tween-20. 1000mL. DNAse Tested. Sterile. 8 pack.
Medchemexpress LLC P62-mediated mitopha 50mg | 1809031-84-2 | 392.15 | C14H9IN4O2 | 50 MG
P62-mediated mitophagy inducer is a small-molecule activator of p62-dependent mitophagy used in research to stimulate mitochondrial autophagy without recruiting Parkin or collapsing mitochondrial membrane potential.
- Activates p62-mediated mitophagy without Parkin recruitment.
- Promotes mitochondrial autophagy while preserving mitochondrial membrane potential.
- Suitable for autophagy and mitophagy research applications.
- Powder form with defined cold-storage stability for long-term storage.
- Available in small research pack sizes for laboratory use.
- Molecular formula C14H9IN4O2; molecular weight 392.15 g·mol⁻¹.